We supply Staurosporine API

We're proud to offer high quality Staurosporine API for our valued guests. it is produced using state- of- the- art manufacturing processes icing harmonious chastity and energy. This important emulsion can be used in the development of new medicines and is generally used in cell biology exploration. We're devoted to furnishing our guests with dependable, cost-effective results to meet their requirements and exceed their prospects. Choose us as your trusted supplier for Staurosporine and let us help you achieve your exploration and medicine development pretensions.

What's Staurosporine?

Staurosporine has natural conditioning ranging from antifungal to antihypertensive. The interest in these natural conditioning has led to expansive exploration in chemistry and biology, as well as the discovery of eventuality foranti-cancer treatments. The main natural exertion of astrosporin is to inhibit protein kinase by precluding the list of ATP and kinase. This is achieved through a stronger affinity of astrosporin for ATP list spots on kinases.

it is a natural product with good medicinal and natural exertion. This emulsion was first insulated by omura in 1997 as an indolecarbazole alkaloid from soil actinomycetes. The analysis ofX-ray single demitasse diffraction trials shows that the center is an indolecarbazole ring, with small patch sugars attached to the n below the ring and an lactam ring attached to the top. Sta is a strong PKC asset( ic50 = 2.7 nm), substantially acting on colorful kinases, topoisomerases, and cell cycle nonsupervisory factors in the cell signaling pathway.

Basic Information

Product Name: Staurosporine

CAS: 62996-74-1

MF:C28H26N4O3

MW:466.54

EINECS:613-127-7

MDL No.:MFCD18252446

Structural formula:

Origin: China

Application: Antibiotics

Delivery Time: in stock

Technical Specification

Package 1g, 5g, 10g, 50g or according to your demands

Storage conditions: Preserve in tightly closed container between 2～8℃

Transportation conditions Transportation at room temperature

Shelf Life 24 months

Biological activity

Staurosporine( CGP 41251, Antibiotic AM- 2282, STS, AM- 2282) is an effective PKC asset that acts on PKC α, PKC γ, and PKC η in cell-free assays with IC50 of 2 nM, 5 nM, and 4 nM, independently. Its effect on PKC δ( 20 nM) and PKC ε( 73 nM) is weak, and its exertion on PKC ε( 1086 nM) is veritably low. At the same time, it also showed inhibitory exertion against other kinases similar as PKA, PKG, S6K, CaMKII, etc. Phase 3.

Target points

Application

It is a natural product that has been extensively studied for its implicit operations in colorful fields. Then are its some common operations:

• Research Tool: It is generally used as a exploration tool in the field of cell biology and biochemistry. It's a broad- diapason protein kinase asset that can inhibit a wide range of protein kinases, making it precious for studying kinase signaling pathways and cellular processes.
• Apoptosis Induction: It is known for its capability to induce apoptosis, or programmed cell death, in a variety of cell types. This property has made it a precious tool for studying apoptosis mechanisms and for probing implicit remedial strategies for cancer treatment.
• Neuroscience Research In neuroscience exploration: It is frequently used to induce neuronal cell death in studies related to neurodegenerative conditions similar as Alzheimer's and Parkinson's complaint. Its capability to induce apoptosis in neuronal cells makes it a precious tool for studying these conditions.
• Anti-Cancer Research: It has shown promisinganti-cancer parcels in preclinical studies. It has been delved for its implicit use in cancer remedy, either alone or in combination with otheranti-cancer agents. Its capability to induce apoptosis in cancer cells makes it a seeker for farther disquisition in cancer treatment.
• Drug Development: It and its derivations have been studied for their eventuality as supereminent composites in medicine development. Experimenters have explored modifying its structure to enhance its particularity, efficacity, and safety for colorful remedial operations.
• Signal Transduction Studies: Its capability to inhibit a wide range of protein kinases makes it precious for studying signal transduction pathways in cells. By blocking specific kinases, experimenters can interpret the places of these enzymes in colorful cellular processes.

Extraction method

This system involves conforming the pH value of the culture medium to 10 with ammonia result after turmoil, and adding n- butyl acetate for birth at room temperature; Add HCl waterless result and transfer the product to the waterless result; Acclimate the pH value to 10 with ammonia result, excerpt with ethyl acetate, and concentrate to gain a brown coagulant; Wash with ether to remove fat; Silica gel chromatography, eluent chloroform methanol, thin- subcaste control, attention, and drying to gain pure product. The chastity reaches over 99%.

In vitro research

it also explosively inhibits HeLaS3 cells, with an IC50 of 4nM. it also inhibits colorful other protein kinases, including PKA, PKG, phosphokinase, S6 kinase, and myosin light chain kinase( MLCK),-- Fgr, Syk and IC50 are 15nM, 18nM, 3nM, 5nM, 21nM, 20nM, 9nM, 6nM, 20nM, 2nM, and 16nM, independently. Staurosporine( 1 μ M) induces over 90 apoptosis in PC12 cells, which can be inhibited by overexpression of Bcl- 2, or by treatment with zVAD fmk, cycloheximide( 10 μ M), and actinomycin D( 5 μ M). similarly, it treatment induces intracellular free calcium situations( Ca) through caspase- 8 activation and shot division, and functional caspase- 3 expression enhances it convinced MCF7 cell death. 1 μ M Staurosporine treatment only incompletely inhibits IL- 3 stimulated Bcl2 phosphorylation, while fully blocking PKC regulated Bcl2 phosphorylation. it induces apoptosis in mortal foreskin fibroblasts AG- 1518, regulated by lysosomal towel protease D for cytochrome c release and caspase activation. In addition to cranking the traditional mitochondrial apoptotic pathway, it triggers a new natural apoptotic pathway that relies on the activation of caspase- 9 in the presence of Apaf- 1.

In vivo research

previous to original anemia,pre-treatment with it(0.1- 10 ng) in original anemia gerbil and rat models can help neuronal damage, and this effect is cure-dependent, indicating that PKC is involved in CAl pyramidal cell death.

Security information

Dangerous goods labeling: Xn, T, Xi

Hazard category code: 40-45-36/37/38-46

Safety instructions: 36/37-53-36-26-25

Dangerous goods transport number: UN281161/PGII

WGKGermany:2

F: 8-10

Hazard level: 3

Customs code: 29419090

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Yihui takes pride in being a trusted Staurosporine manufacturer and supplier. To acquire this groundbreaking product or discuss your specific requirements, contact us immediately at sales@yihuipharm.com. Rest assured, with Yihui, you're accessing a product of unparalleled quality, backed by certifications that underscore our commitment to excellence in the global market.